e-nnovation Life Sciences offers a variety of probe and primer for real- time qPCR, including dual-labelled BHQ ® probes, BHQplus® probes, Molecular Beacons, and Scorpion® primers.

DNA Quantification & Gene Expression Analysis

RealTime Design Software ensures robust amplification and detection

While designing probes to quantify DNA for copy number studies (gDNA) or gene expression analysis (cDNA), traditional dual-labeled probes quenched with the BHQ dye are more than sufficient. However, many difficult target sites are AT- rich, and so fortified BHQplus probes are required to maintain an elevated melting temperatures.

BHQplus probes provide more flexibility in design of an assay for DNA quantification, these shorter probes can be accommodated into a greater variety of targets and with a wider range in base composition.

With the “Quantification PCR” module of Real Time Design software, probes can be designed for sensitive measurements of copy numbers.

• Choose BHQ or BHQ plus probes partnered with the ideal fluorophore for the instrument optics in use.

• Use NCBI gene symbols, RefSeq IDs, or GenBank accession numbers, or use your own optimized sequence.

• Pick the optimal assays designed by Real Time Design software or choose alternate primers and probes using Custom Mode.

• Order directly through the Biosearch website.

 

Probe-Based SNP Genotyping

Real Time Design for Allelic Discrimination

Because the BHQplus technology stabilizes probe hybridization to the template, it allows for the design of shorter probes that offer increased resistance against hybridizing to a mismatch. This makes BHQplus probes ideal for genotyping applications such as screening, linkage associations, or fine-mapping studies. Designing optimal probes is simple with Real Time Design’s “SNP Genotyping” module (found under qPCR- BHQ probe > Application > SNP Genotyping)

qPCR & SNP Genotyping