Product quality is of utmost importance to us. we are acutely aware of the time, effort and money our customers invest in their work. Poor quality reagents can ruin even the best-designed experiments. Our commitment to quality is a direct result of this awareness. From the receiving dock to the shipping dock to your lab bench, we have developed exhaustive quality assurance protocols that test and document each important physical parameter of every compound that enters and leaves our labs. Starting with the basic raw materials and ending with the final highly-purified end product, we maintain purity and quality documentation that ships with every product we sell.
Q-PCR & SNP Genotyping
Dual-Labeled BHQ® Probes
View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC Biosearch. We synthesize high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.
BHQnova Probes
The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnovaTM probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.
An advanced technology for qPCR and SNP genotyping, the BHQplus probe is a compact oligo fortified with our signature duplex stabilizing chemistry to elevate the melting temperature and enhance target specificity. BHQplus probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.
Molecular Beacons
Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC Biosearch currently offers Molecular Beacons labeled with either a Black Hole Quencher® or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.
Scorpions Primers
Scorpions® primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC Biosearch’s selection of Scorpions primers.
Calibration Dyes
Do you intend to multiplex qPCR assays using FAM, CAL Fluor® and/or Quasar® dyes? Remember to optimize your instrument’s performance by using the calibration standards available from LGC Biosearch.
SuperROX is a highly stable passive reference dye that is formulated to significantly reduce “ROX drop.” It can be used when a water soluble version of ROX is required as a passive reference to normalize fluorescent signals in real-time PCR.
ValuPanel Reagents
ValuPanel Reagents are composed of pre-designed probes and primers that are intended to resolve the genetic signatures to of different pathogen strains. They are for Research Use Only (RUO), not for use in diagnostic procedures.
RNA FISH
Custom Probe Sets
Stellaris Custom RNA FISH probe sets can be designed against almost any RNA target to allow for visualization, localization, and quantification of individual mRNA transcripts.
ShipReady Probe Sets
Stellaris ShipReady RNA FISH probe sets provide convenient, ready-to-ship positive controls for reference and long non-coding RNA targets.
DesignReady Probe Sets
Stellaris DesignReady probe sets are professionally designed, and go through rigorous bioinformatic analysis to ensure specificity. Stellaris DesignReady probe sets are made-to-order.
RNA FISH Buffers
Stellaris Buffers are ideal for performing Stellaris RNA FISH on cells and tissue, as well as other sample types. The buffers contain proprietary additives to enhance signal and reduce background in assays that may typically exhibit more pronounced background fluorescence
Chromatin Isolation via RNA Precipitation (ChIRP)
ChIRP is a rapid technique to discover RNA-associated DNA sequences and map genomic binding sites of chromatin associated long noncoding RNAs (lncRNAs) with high sensitivity and low background.
